Mouse & Rat Microsurgical Techniques - Agenda
Course 1 - Day 1 (May 7, 2008)
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8:00 - 8:30 |
Coffee and Introductions |
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8:30 - 9:00 |
Review of microscopes: Objectives, ocular, depth of field, field of vision, lighting sources
Surgical instruments: List of necessary instruments, uses and non-uses of instruments, care and cleaning, sterilizing surgical packs |
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9:00 - 10:00 |
Review of chronic surgery: Aseptic technique, sterilization methods, suturing, catheter material and manufacture
Anesthetics and planes of surgical anesthesia, monitoring, analgesics, respirators & ACUC requirements |
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10:00 - 10:30 |
Coffee Break |
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10:30 - 11:00 |
Practice Session: Suturing techniques, latex simulation |
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11:00 - 12:00 |
Demonstration of the following:
i) Intubation of rats and mice
ii) Isolation of vessels in mice
iii) Femoral artery blood flow and pressure; computerized data collection |
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12:00 - 1:00 |
Lunch provided |
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1:00- 4:30 |
Practical Session: Isolation of carotid artery, jugular vein, femoral vessels, etc. |
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4:30- 5:00 |
Questions & Answers session |
Course 1 - Day 2 (May 8, 2008)
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8:00 - 8:30 |
Coffee and Discussion of Previous Day |
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8:30 - 10:00 |
Demonstration:
Mouse Ascending Aorta Flow Probe Implantation |
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10:00 - 10:30 |
Coffee Break |
|
10:30 - 12:00 |
Practical Session: Ascending Aorta Flow Probe
(Mouse or Rat) |
|
12:00 - 1:00 |
Lunch – provided |
|
1:00 – 2:00 |
Demonstration:
Mouse/Rat Renal Artery Flow Probe |
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2:00 - 4:30 |
Practical Session: Participant's choice |
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4:30 - 5:00 |
Questions & Answers |
Course 1 - Day 3 (May 9, 2008)
|
8:00 - 8:30 |
Coffee and Discussion of Previous Day |
|
8:30 - 9:30 |
Demonstration: Cardiac pressure/volume loops |
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9:30- 10:00 |
Practical: Participant's choice |
|
10:00 - 10:30 |
Coffee Break |
|
10:20 - 12:00 |
Practical: Participants Choice
Demonstration: Mouse/Rat renal flow probe implantation |
|
12:00 - 1:00 |
Lunch - provided |
|
1:00 - 4:30 |
Practical: Participant's choice |
|
4:30 - 5:00 |
Questions & Answers |
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